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IgM Capture Bestarose FF is a sulfur-philic affinity resin made by fixing sulfur-containing compound 2-mercaptopyridine on fine particles of high rigidity agarose. Its optimized ligand density and IgM have suitable affinity, and fine particle microspheres can increase the binding capacity of IgM with larger molecular weight. The principle of sulfur affinity is to use the interaction between the electron donor and the electron acceptor to separate and purify biomolecules. This force is strengthened in a high-salt environment and weakened in a low-salt environment.
|
Appearance |
White slurry |
|
Matrix |
Highly cross-linked agarose, 6% |
|
Particle size+ |
45~165μm |
|
Functional group |
2-mercaptopyridine |
|
Ligand concentration |
~ 2mg 2-mercaptopyridine /mL resin |
|
Chemical stability |
Stable in common aqueous buffers:70% ethanol, 30% isopropyl alcohol, 1M HAc++, 0.1M NaOH |
|
Pressure flow velocity |
250~400cm/h（0.1MPa BXK50/30, H=25cm, 20℃） |
|
Max. pressure |
0.3MPa |
|
pH stability |
3~11(working),2~13(CIP) |
|
Storage+++ |
2~30℃,20% ethanol or 2% benzyl alcohol |
+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.
++ 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
IgM Capture Bestarose FF |
AA0172 |
25mL |
|
AA0173 |
100mL |
|
|
AA0174 |
500mL |
|
|
AA0175 |
1L |
|
|
AA0176 |
5L |
|
|
AA0177 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast IgM FF |
EA214301 |
1×1mL |
|
EA042 |
5×1mL |
|
|
EH214303 |
1×5mL |
|
|
EA043 |
5×5mL |
|
|
EzScreen IgM FF |
EA01725 |
1×4.6mL |
|
EA01735 |
5×4.6mL |
|
|
EzLoad 16/10 IgM FF |
EA214304 |
1 pcs |
|
EzLoad 26/10 IgM FF |
EA214306 |
1 pcs |
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