English
Request a Quote
Ni Bestarose HP (High Performance) metal chelation chromatography resin is an affinity chromatography resin made by pre-chelating metal ion Ni2+ on a high-resolution agarose with NTA as ligand. It has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.
|
Appearance |
Turquoise slurry |
|
Matrix |
Cross-linked agarose, 6% |
|
Particle size+ |
24~44μm(the average particle size is 34μm) |
|
Ligand concentration |
~15μmol Ligand/mL resin |
|
Dynamic binding capacity |
~ 40mg His tag protein/mL packed resin |
|
Chemical stability++ |
1 week at 40℃: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl 40℃ 12h: 1M NaOH, 70% acetic acid |
|
Max. pressure |
0.3MPa |
|
pH stability+++ |
3~12(working) 2~14(CIP) |
|
Storage++++ |
2~30℃,20% ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
＜150cm/h |
+Particle size is the cumulative resin particle size of packing volume distribution.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Ni Bestarose HP |
AA0041 |
25mL |
|
AA0042 |
100mL |
|
|
AA0043 |
500mL |
|
|
AA0044 |
1L |
|
|
AA0045 |
5L |
|
|
AA0046 |
10L |
|
|
AA208115 |
20L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Ni HP |
EA208101 |
1×1mL |
|
EA208103 |
1×5mL |
|
|
EA004 |
5×1mL |
|
|
EA005 |
5×5mL |
|
|
EzScreen Ni HP |
EA00425 |
1×4.6mL |
|
EA00435 |
5×4.6mL |
|
|
EzLoad 16/10 Ni HP |
EA208104 |
1 pcs |
|
EzLoad 26/10 Ni HP |
EA208106 |
1 pcs |
|
EzLoad 26/20 Ni HP(Double adaptor） |
EA00414 |
1 pcs |
.png)
.png)
.png)
.png)
.png)
